Cleaner Gene Editing With Cas-CLOVER

Our Cas-CLOVER gene editing technology helps eliminate off-target mutations and is available through simple licenses.


Our Cas-CLOVER gene editing technology has undetectable off-target activity and stand alone patent protection for licensing simplicity and clarity in freedom to operate.

Evaluate Cas-CLOVER in-house or collaborate with us to execute your proof of concept services. Then, partner with us for accessible commercial and research licenses for the areas of agriculture, industrial biotechnology and pharmaceutical bioprocessing for a more rewarding gene editing experience.


Cas-CLOVER Is More Precise Than CRISPR

Cas-CLOVER is a hybrid gene editing system using a nuclease-inactivated Cas9 protein fused to a Clo51 endonuclease.​

dCas9 serves as a fusion protein only. It is mutated and unable to cut DNA. As opposed to the use of a single guide RNA (gRNA) like in CRISPR, the Cas-CLOVER system utilizes two gRNAs in addition to the Clo51 nuclease activity that requires dimerization of subunits associated with each guide RNA. This allows for more stringent DNA cleavage. Using two gRNA makes the Cas-CLOVER gene editing system highly restricted and only functional when the paired gRNAs dimerize at the correct target site.

The specificity of Cas-CLOVER also enables multiple rounds of targeting at one locus to increase indel frequency without increased risk of unwanted off-target mutations.

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Cas-CLOVER Is Simple To License

Current gene editing tools, particularly CRISPR, are held back by confusing intellectual property restrictions. This lack of commercial freedom to operate has limited CRISPR’s applications.

It is our mission to make technology easily accessible. Due to the distinct science involved, Cas-CLOVER is covered under a set of patents separate from other technologies and is easy to license.

It’s exclusively licensed and sublicensed by Demeetra AgBio for agriculture, synthetic biotechnology, and pharmaceutical bioprocessing.

Validated In CHO Cells, Yeast and Plants

Cas-CLOVER is advancing research across multiple industries today.

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Pharmaceutical Bioprocessing

Bioprocessing and cell line engineering to produce human or non-human therapeutics

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Synthetic Biotechnology

Bioprocessing and strain improvement to produce therapeutics, industrial enzymes, compounds or biofuels

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Agriculture Biotechnology

Enable plant modifications that may not require GMO labels and for the production of novel therapeutics

How Cas-CLOVER Works

View the specifics of Cas-CLOVER’s mechanism.

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Easily designed and deployed reagents

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Fast, dCas9-guided sequence recognition

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2 gRNAs match target and subunit dimerizes before cleavage


Shop Gene Editing Reagents Online

Buy reagents for use in plant, yeast, and agricultural animal gene editing by visiting our easy-to-use shop page. After purchasing the vials and signing a simple royalty-free MTA form, we’ll send you our basic use protocol, which includes maps, sequences, instructions on design and production of the gRNAs.


We utilize Cas-CLOVER as our go-to gene editing technology for many reasons. However, the requirement of a GG “PAM” binding site is technically a constraint on Cas-CLOVER and other gRNA-dependent biotechnologies like CRISPR-Cas9.

TAL-CLOVER remedies this since it only requires a “T” anchor.


New TAL-CLOVER: More Flexible Than CRISPR and gRNA-based Gene Editing

Related Resources

Check out our latest content on Cas-CLOVER.

Scientists Trust Our Gene Editing Technology


“We are very happy with the efficiency of the targeted mutations we are getting with Cas-CLOVER in mammalian cells. It is impressive that although Cas-CLOVER requires dimerization it still has very high efficiency similar to CRISPR/Cas9. The one aspect of Cas-CLOVER that stood out for us was its ease of use. We have been working with CRISPR/Cas9 for many year now and Cas-CLOVER just fit in very nicely with our existing workflow for gene-editing with Cas9 or Cas9 nickase.”


Tseten Jamling, PhD.

Head of Molecular Biology
Bristol Myers Squibb

Kayla Bean

On my experience using cas-CLOVER…the uniqueness adds to the specificity of the system.“The flexibility of the guide RNA design makes the system easy to use and gives high specificity due to the use of the two guide RNAs. It is very efficient due to the ability of the dead Cas9s to recognize the current area of DNA, and since the clo51 nuclease can only cut when dimerized, the system has high fidelity.


Kayla Bean, Ph.D.

Research Scientist, Discovery Research
Elanco Animal Health

We Can Help Optimize Your Research

Contact us to learn more about Cas-CLOVER can help accelerate your research.