Cleaner Gene Editing With Cas-CLOVER™
Our Cas-CLOVER gene editing technology helps eliminate off-target mutations, has been validated in plants, mammalian cells and yeast and is available through simple licenses.
The Cas-CLOVER gene editing technology has undetectable off-target activity and stand alone patent protection for licensing simplicity and clarity in freedom to operate.
Evaluate Cas-CLOVER, with no strings attached, in-house or collaborate with us to execute your proof of concept studies. Then, if you choose, partner with us for accessible commercial focused licenses for the areas of agriculture (plants & animals), industrial biotechnology (yeast) and pharmaceutical bioprocessing (mammalian cells) for a more rewarding gene editing experience.
High Efficiency, Flexible Design, Larger Deletions & Simpler Screening
Cas-CLOVER’s dCas serves as a fusion protein only. It is mutated and unable to cut DNA. As opposed to the use of a single guide RNA (gRNA) like in CRISPR, the Cas-CLOVER system utilizes two gRNAs in addition to the Clo051 nuclease activity that requires dimerization of subunits associated with each guide RNA. This allows for more stringent DNA cleavage. Using two gRNA makes the Cas-CLOVER gene editing system highly targeted and only functional when the paired gRNAs dimerize at the correct target site.
The image shows ultra-low potential off-targets in an unbiased iGUIDE-seq study presented at the Poseida Therapeutics R&D day 2021.
Tobacco is tetraploid, accordingly 4 copies of target genes must be deleted to render a full knockout. Here is an example of tobacco editing efficiency with Cas-CLOVER, where 0%= no cutting, 25%=one allele, 50%=two alleles, 75%=3 alleles and 100%=all 4 alleles cut. The vast majority of the 70+ individual plants have between 2-4 alleles knocked out.
Flexibility for Choosing Target Sequence
Clo051 nuclease allows for a flexible spacer region of 15–30 base-pairs between guides. Cas-CLOVER requires two gRNAs to function but the flexibility of Clo051 enables simple target designs for multiple loci in any gene.
Another advantage of Cas-CLOVER’s two gRNA targeting system is it generally results in larger deletions around 8-50 base-pairs (bp) to CRISPR/Cas9’s 1-3 bps. Edited cells, yeast or plants can thus be positively identified by running quick and easy gels in-house; streamlining your workflows and saving the time and resources, instead of sending samples out for sequencing.
Simple To License
Current gene editing tools, particularly CRISPR, are held back by confusing intellectual property restrictions. This lack of commercial freedom to operate has limited CRISPR’s applications.
It is our mission to make technology accessible. Due to the distinct science involved, Cas-CLOVER is covered under a set of patents separate from other technologies and is easy to license.
It’s exclusively licensed and sublicensed by Demeetra AgBio for agriculture, synthetic biotechnology, and pharmaceutical bioprocessing. Start your evaluation now by starting the process of ordering reagents today.
Validated In CHO Cells, Yeast and Plants
We specialize in multiple industries, allowing us to apply Cas-CLOVER and piggyBac
technologies into broad real world applications.
Bioprocessing and cell line engineering to produce human or non-human therapeutics
Bioprocessing and strain improvement to produce therapeutics, industrial enzymes, compounds or biofuels
Enable plant modifications that may not require GMO labels and for the production of novel therapeutics
How Cas-CLOVER Works
View the specifics of Cas-CLOVER’s mechanism.
Easily designed and deployed reagents
Fast, dCas-guided sequence recognition
2 gRNAs match target and subunit dimerizes before cleavage
Explore Gene Editing Reagents Online
Select reagents for use in plant, yeast, and animal genomes by visiting our easy-to-use catalog page. After requesting a quote and signing a simple royalty-free MTA form, you will receive our basic use protocol - which includes maps; sequences; and instructions on the design and production of gRNAs.
TAL-CLOVER vs. Cas-CLOVER
We utilize Cas-CLOVER as our go-to gene editing technology for many reasons. However, the requirement of a GG “PAM” binding site is technically a constraint on Cas-CLOVER and other gRNA-dependent biotechnologies like CRISPR-Cas9.
TAL-CLOVER remedies this since it only requires a “T” anchor.
New TAL-CLOVER: More Flexible Than CRISPR and gRNA-based Gene Editing
Check out our latest content on Cas-CLOVER.
Scientists Trust Our Gene Editing Technology
“We are very happy with the efficiency of the targeted mutations we are getting with Cas-CLOVER in mammalian cells. It is impressive that although Cas-CLOVER requires dimerization it still has very high efficiency similar to CRISPR/Cas9. The one aspect of Cas-CLOVER that stood out for us was its ease of use. We have been working with CRISPR/Cas9 for many year now and Cas-CLOVER just fit in very nicely with our existing workflow for gene-editing with Cas9 or Cas9 nickase.”
Tseten Jamling, PhD.
Head of Molecular Biology
Bristol Myers Squibb
On my experience using cas-CLOVER…the uniqueness adds to the specificity of the system.“The flexibility of the guide RNA design makes the system easy to use and gives high specificity due to the use of the two guide RNAs. It is very efficient due to the ability of the dead Cas9s to recognize the current area of DNA, and since the clo51 nuclease can only cut when dimerized, the system has high fidelity.
Kayla Bean, Ph.D.
Research Scientist, Discovery Research
Elanco Animal Health