Best-In-Class Gene Editing With Cas-CLOVER™ & piggyBac®
Try Cas-CLOVER and piggyBac together for Footprint-Free® Gene Editing.
Cas-CLOVER can be combined with piggyBac for Footprint-Free Gene Editing. Cas-CLOVER is our exciting gene editing technology that functions as a cleaner alternative to CRISPR/Cas9. piggyBac is proven for stable gene integration and seamless excision with 750+ appearances in peer-reviewed journals.
Footprint-Free Gene Editing, Version 1
Step 1: With Cas-CLOVER, generate a double stranded break in the genomic DNA next to the desired gene edit. Using homologous recombination (HR) insert the desired gene edit along with a positive/negative selectable marker flanked by the piggyBac target sequences, inverted terminal repeats (ITRs).
Step 2: Select for the targeted integration of your desired gene edit, increasing efficiency of a sometimes very rare event by killing off all non-edited cells.
Step 3: Introduce excision-only piggyBac transposase which seamlessly removes the selectable marker leaving behind a correctly edited cell with no other footprints.
Footprint-Free Gene Editing, Version 2
Step 1: Stably introduce Cas-CLOVER into the cell or organism of choice using piggyBac transposase.
Step 2: The stable integration of Cas-CLOVER increases expression and editing efficiency.
Step 3: Introduce the gRNA and/or donor vectors for gene editing.
Step 4: Excision-only piggyBac is used to stably remove Cas-CLOVER, leaving behind the desired edit without a trace.
Used in CHO, Yeast, and Plant Cells
Both Cas-CLOVER and piggyBac gene editing technologies are advancing research across multiple industries today.
Bioprocessing and cell line engineering to produce human or non-human therapeutics
Bioprocessing and strain improvement to produce therapeutics, industrial enzymes, compounds or biofuels
Enable plant modifications that may not require GMO labels and for the production of novel therapeutics