Simple Commercial Access To Powerful Precision Cas-CLOVER™ Gene Editing And Stable piggyBac® Protein Production Technologies
Browse and select Cas-CLOVER and piggyBac reagents, or schedule a call if you would like to speak to an expert team member. Our technology is available for license fee-free research evaluation followed by commercial use options with full freedom to operate (FTO). Backed by a multi-institutional, globally issued intellectual property portfolio.
Demeetra's Fields of Use include Bioprocessing, Synthetic Biotechnology and Agriculture. Simply request Cas-CLOVER or piggyBac reagents below. We will send a quote, a simple evaluation agreement, protocols and can provide expert support to your team
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Mammalian Gene Editing
Plant Gene Editing
Yeast Gene Editing
Mammalian Gene Editing
| SKU # | TECHNOLOGY | DESCRIPTION | RESOURCE | PRICE | + |
|---|---|---|---|---|---|
| rDMT5-011 | Cas-CLOVER mRNA | Cas-CLOVER mRNA: Codon optimized mRNA for mammalian systems. Use our gRNA pair design overview or Demeetra can custom design the target pairs for you (contact us below). Order gRNAs from your favorite provider and transform with the mRNA for high efficiency and clean editing. Components: 1 vial containing 25µg of mRNA | Design Guide | $650 | |
| rDMT5-027 | piggyBac | Super piggyBac Transposase mRNA:Codon optimized for mammalian systems. Transform cells with piggyBac mRNA and piggyBac transposon (pDMT5-028) for stable integration. Use for any cell of interest for biomanufacturing, food products, agriculture and synthetic biology Components: 1 vial containing 25µg of mRNA | User Guide | $650 | |
| rDMT5-020 | piggyBac | Excision-Only piggyBac Transposase mRNA:Use Excision-only piggyBac transposase to remove previously integrated piggyBac transposons for phenotype reversions and Footprint-Free gene editing.
Components: 1 vial containing 25µg of mRNA | User Guide | $650 | |
| pDMT5-029 | piggyBac | Super piggyBac Transposase Plasmid:Codon optimized for mammalian systems. Transform cells with piggyBac plasmid and piggyBac transposon (pDMT5-028) for stable integration. Use for any cell of interest for biomanufacturing, food products, agriculture and synthetic biology. Please note this plasmid is not expandable.
Components: 1 vial containing 25µg of plasmid DNA | Map
User Guide | $650 | |
| pDMT5-030 | piggyBac | Excision-Only piggyBac Transposase DNA:Use Excision-only piggyBac transposase to remove previously integrated piggyBac transposons for phenotype reversions and Footprint-Free gene editing. Please note this plasmid is not expandable.
Components: 1 vial containing 25µg of plasmid DNA | Map
User Guide | $650 | |
| pDMT5-028 | piggyBac | piggyBac Transposon:Using the flexible MCS, clone in your promoter, gene and other components for optimal expression in your system. Includes ampicillin resistance.
Components: 1 vial containing 10µg of plasmid DNA | Map | $350 |
Plant Gene Editing
Plant Cas-CLOVER Gene Editing Plasmid Vector Suite: Codon optimized T-DNA based plant transformation vectors for gene knockout in plants using the Cas-CLOVER system. Expression promoters for Cas-CLOVER, gRNA’s, selection markers and other details are listed below along with links to the maps.
All vectors are 5µg per vial.
| PLASMID SKU# | CODON OPTIMIZATION | CAS-CLOVER PROMOTER | GRNA PROMOTER | HOST SELECTION | BACTERIAL SELECTION | PLASMID NOTES | MAP LINK | PRICE | + |
|---|---|---|---|---|---|---|---|---|---|
| pDMT1-1 | A. thaliana | CaMV35S | OsU3 | Hygromycin | Kanamycin | Sequential cloning of gRNAs | Map | $500 | |
| pDMT3-4 | A. thaliana | CaMV35S | AtU6-26 | Hygromycin | Kanamycin | Sequential cloning of gRNAs | Map | $500 | |
| pDMT1-123 | A. thaliana | CaMV35S | OsU6 | Hygromycin | Kanamycin | Sequential cloning of gRNAs | Map | $500 | |
| pDMT1-112 | A. thaliana | CaMV35S | OsU3 | Kanamycin | Kanamycin | Sequential cloning of gRNAs | Map | $500 | |
| pDMT1-114 | A. thaliana | CaMV35S | AtU6-26 | Kanamycin | Kanamycin | Sequential cloning of gRNAs | Map | $500 | |
| pDMT1-116 | A. thaliana | CaMV35S | OsU3 | Glufosinate | Kanamycin | Sequential cloning of gRNAs | Map | $500 | |
| pDMT1-118 | A. thaliana | CaMV35S | AtU6-26 | Glufosinate | Kanamycin | Sequential cloning of gRNAs | Map | $500 |
Yeast Gene Editing
Yeast Cas-CLOVER Gene Editing Vector Suite: We offer a series of yeast-based shuttle vectors for the expression of a Saccharomyces cerevisiae codon optimized version of Cas-CLOVER. In this platform we have designed the plasmids such that gRNA expression is polyscistronic. Expression of the Cas-CLOVER is driven by ScRNR2 promoter, and individual guides are expressed by ScSNR52 and are liberated via an intervening tRNA.
| PLASMID SKU# | CODON OPTIMIZATION | CAS-CLOVER PROMOTER | GRNA PROMOTER | HOST SELECTION | HOST REPLICATION | BACTERIAL SELECTION | PLASMID NOTES | MAP LINK | PRICE | + |
|---|---|---|---|---|---|---|---|---|---|---|
| pDMT1-124 | S. cerevisiae | ScRNR2 | No Guide Expression Cassette | LEU2 | CEN/ARS | Ampicillin | BsaI sites have been mutated | Map | $500 | |
| pDMT1-125 | S. cerevisiae | ScRNR2 | ScSNR52 | LEU2 | CEN/ARS | Ampicillin | Two guides can be cloned via GGA | Map | $500 | |
| pDMT1-126 | S. cerevisiae | ScRNR2 | ScSNR52 | URA3 | CEN/ARS | Ampicillin | Two guides can be cloned via GGA | Map | $500 | |
| pDMT1-127 | S. cerevisiae | ScRNR2 | ScSNR52 | KanR | CEN/ARS | Ampicillin | Two guides can be cloned via GGA | Map | $500 | |
| pDMT1-128 | S. cerevisiae | ScRNR2 | ScSNR52 | HygR | CEN/ARS | Ampicillin | Two guides can be cloned via GGA | Map | $500 |
Yeast piggyBac Engineering Vector Suite: All vectors are 10µg of plasmid DNA.
| PLASMID SKU# | CODON OPTIMIZATION | DESCRIPTION | MAP LINK | PRICE | + |
|---|---|---|---|---|---|
| pDMT3-23 | S. cerevisiae | Taking advantage of the unique massive cargo capacity, this ~25kb piggyBac transposon vector contains all genetic components required for engineering the yeast mevalonate pathway (MVA) in a single-step for the production of a high value secondary metabolites. View data. | Map | $1998 | |
| pDMT4-1 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-1 has a native URA3 promoter driving a URA3 auxotrophic marker. | Map | $650 | |
| pDMT4-2 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-2 has a defective URA3 promoter driving a URA3 auxotrophic marker allowing for more potential integrations of the transposon cassette. | Map | $650 | |
| pDMT4-3 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-3 has a native LEU2 promoter driving a LEU2 auxotrophic marker. | Map | $650 | |
| pDMT4-4 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-4 has a defective LEU2 romoter driving a LEU2 auxotrophic marker allowing for more potential integrations of the transposon cassette. | Map | $650 | |
| pDMT4-5 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-5 has a native HIS3 promote driving a HIS3 auxotrophic marker. | Map | $650 | |
| pDMT4-6 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-6 has a defective HIS3 promoter driving a HIS3 auxotrophic marker allowing for more potential integrations of the transposon cassette | Map | $650 | |
| pDMT4-7 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-7 has a native TRP1 promoter driving a TRP1 auxotrophic marker. | Map | $650 | |
| pDMT4-8 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-8 has a defective TRP1 promoter driving a TRP1 auxotrophic marker allowing for more potential integrations of the transposon cassette. | Map | $650 |
GGA = Golden Gate Assembly
Total Reagents: 0
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Demeetra's Fields of Use include Bioprocessing, Synthetic Biotechnology and Agriculture. Simply request Cas-CLOVER or piggyBac reagents below. We will send a quote, a simple evaluation agreement, protocols and can provide expert support to your team
Cell-Based Bioprocessing
Cell line development, cultured meats and protein production
Agriculture
Advanced crop trait engineering, the non-GMO way
Synthetic Biotechnology
Harnessing the power of targeted yeast editing and stable expression
Enhance your gene editing research interests with Cas-CLOVER. The recorded webinars hosted by Genetic Engineering & Biotechnology News are available below so that you can watch the areas that apply to your research.
Tseten Jamling, PhD.
Scientist, BMS
Maximilian Richter, PhD.
Senior Scientist, Immuno-Oncology, Poseida Therapeutics
Contact us to learn more about our gene editing technology.