Our Powerful Gene Editing Toolbox Of Precision Cas-CLOVER™ And Flexible piggyBac® Gene Insertion Technologies
Browse Cas-CLOVER and piggyBac reagents, now available for risk-free research evaluation and or commercial applications with full freedom to operate (FTO).
Browse Reagents
In order to facilitate commercial development in the fields of Pharmaceutical Bioprocessing, Synthetic Biotechnology and Agricultural Biotechnology, we have removed barriers to entry typical of other gene editing technologies.
Our technology evaluation licensing process is risk free.
Simply purchase Cas-CLOVER or piggyBac reagents below and accept the simple evaluation agreement (which can also be downloaded and shared). We will provide our standard user protocols, which includes plasmid maps, instructions on design and production of vectors and gRNAs.
1. Order Reagents
2. Complete In-House Or Outsource Evaluation
3. Get A Commercial License
Mammalian Gene Editing
Mammalian-Optimized
Cas-CLOVER mRNA
| SKU# | Technology | Description | Resource | Price |
|---|---|---|---|---|
| rDMT5-011 | Cas-CLOVER | Cas-CLOVER mRNA: Codon optimized research grade mRNA for mammalian systems. Use our gRNA pair design overview or Demeetra can custom design the target pairs for you (contact us below). Order gRNAs from your favorite provider and transform with the mRNA for high efficiency and clean editing. Components: 1 vial containing 25µg of mRNA | Design Guide | $650 |
Mammalian piggyBac Reagents
| SKU# | Technology | Description | Resource | Price |
|---|---|---|---|---|
| rDMT5-027 | piggyBac | Super piggyBac transposase mRNA: Codon optimized for mammalian systems. Transform cells with piggyBac mRNA and piggyBac transposon (pDMT5-028) for stable integration. Use for any cell of interest for biomanufacturing, food products, agriculture and synthetic biology. Components: 1 vial containing 25µg of mRNA | User Guide | $650 |
| rDMT5-020 | piggyBac | Excision-only piggyBac transposase mRNA: Use Excision-only piggyBac transposase to remove previously integrated piggyBac transposons for phenotype reversions and Footprint-Free gene editing. Components: 1 vial containing 25µg of mRNA | User Guide | $650 |
| pDMT5-029 | piggyBac | Super piggyBac transposase plasmid: Codon optimized for mammalian systems. Transform cells with piggyBac plasmid and piggyBac transposon (pDMT5-028) for stable integration. Use for any cell of interest for biomanufacturing, food products, agriculture and synthetic biology. Please note this plasmid is not expandable. Components: 1 vial containing 25µg of plasmid DNA | Map User Guide | $650 |
| pDMT5-030 | piggyBac | Excision-only piggyBac transposase DNA: Use Excision-only piggyBac transposase to remove previously integrated piggyBac transposons for phenotype reversions and Footprint-Free gene editing. Please note this plasmid is not expandable. Components: 1 vial containing 25µg of plasmid DNA | Map User Guide | $650 |
| pDMT5-028 | piggyBac | piggyBac transposon: Using the flexible MCS, clone in your promoter, gene and other components for optimal expression in your system. This plasmid is expandable. Components: 1 vial containing 10µg of plasmid DNA | Map | $350 |
Cas-CLOVER gRNA Design Services
Cas-CLOVER gRNA Design Services
SKU: D003
Expert consultation and design deliverable for one pair (2x gRNAs) using state-of-the-art methods gene editing with Cas-CLOVER
1 Pair (2x gRNAs designed)
Plant Gene Editing
Plant Cas-CLOVER Gene Editing Plasmid Vector Suite
Codon optimized T-DNA based plant transformation vectors for gene knockout in plants using the Cas-CLOVER system. Expression promoters for Cas-CLOVER, gRNA’s, selection markers and other details are listed below along with links to the maps.
All vectors are 5µg vial
| Plasmid Sku# | Codon Optimization | Cas-CLOVER Promoter | gRNA Promoter | Host Selection | Bacterial Selection | Plasmid Notes | Map link | Price |
|---|---|---|---|---|---|---|---|---|
| pDMT1-1 | A. thaliana | CaMV35S | OsU3 | Hygromycin | Kanamycin | Sequential cloning of gRNAs | Map | $500 |
| pDMT3-4 | A. thaliana | CaMV35S | AtU6-26 | Hygromycin | Kanamycin | Sequential cloning of gRNAs | Map | $500 |
| pDMT1-123 | A. thaliana | CaMV35S | OsU6 | Hygromycin | Kanamycin | Sequential cloning of gRNAs | Map | $500 |
| pDMT1-112 | A. thaliana | CaMV35S | OsU3 | Kanamycin | Kanamycin | Sequential cloning of gRNAs | Map | $500 |
| pDMT1-114 | A. thaliana | CaMV35S | AtU6-26 | Kanamycin | Kanamycin | Sequential cloning of gRNAs | Map | $500 |
| pDMT1-116 | A. thaliana | CaMV35S | OsU3 | Glufosinate | Kanamycin | Sequential cloning of gRNAs | Map | $500 |
| pDMT1-118 | A. thaliana | CaMV35S | AtU6-26 | Glufosinate | Kanamycin | Sequential cloning of gRNAs | Map | $500 |
GGA=Golden Gate Assembly
Yeast Gene Editing
Yeast Cas-CLOVER Gene Editing
Vector Suite
We offer a series of yeast-based shuttle vectors for the expression of a Saccharomyces cerevisiae codon optimized version of Cas-CLOVER. In this platform we have designed the plasmids such that gRNA expression is polyscistronic. Expression of the Cas-CLOVER is driven by ScRNR2 promoter, and individual guides are expressed by ScSNR52 and are liberated via an intervening tRNA.
| Plasmid Sku# | Codon Optimization | Cas-CLOVER Promoter | gRNA Promoter | Host Selection | Host Replication | Bacterial Selection | Notes About Plasmid | Map link | Price |
|---|---|---|---|---|---|---|---|---|---|
| pDMT1-124 | S. cerevisiae | ScRNR2 | No Guide Expression Cassette | LEU2 | CEN/ARS | Ampicillin | BsaI sites have been mutated. | Map | $500 |
| pDMT1-125 | S. cerevisiae | ScRNR2 | ScSNR52 | LEU2 | CEN/ARS | Ampicillin | Two guides can be cloned via GGA | Map | $500 |
| pDMT1-126 | S. cerevisiae | ScRNR2 | ScSNR52 | URA3 | CEN/ARS | Ampicillin | Two guides can be cloned via GGA | Map | $500 |
| pDMT1-127 | S. cerevisiae | ScRNR2 | ScSNR52 | KanR | CEN/ARS | Ampicillin | Two guides can be cloned via GGA | Map | $500 |
| pDMT1-128 | S. cerevisiae | ScRNR2 | ScSNR52 | HygR | CEN/ARS | Ampicillin | Two guides can be cloned via GGA | Map | $500 |
GGA=Golden Gate Assembly
Yeast piggyBac Engineering Vector Suite
| Plasmid SKU# | Codon Optimization | Description | Map link | Price |
|---|---|---|---|---|
| pDMT3-23 | S. cerevisiae | Taking advantage of the unique massive cargo capacity, this ~25kb piggyBac transposon vector contains all genetic components required for engineering the yeast mevalonate pathway (MVA) in a single-step for the production of a high value secondary metabolites. This plasmid is expandable. View data<.a>. Components: 1 vial containing 10µg of plasmid DNA | Map | $1998 |
| pDMT4-1 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-1 has a native URA3 promoter driving a URA3 auxotrophic marker. This plasmid is expandable Components: 1 vial containing 10µg of plasmid DNA | Map | $650 |
| pDMT4-2 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-2 has a defective URA3 promoter driving a URA3 auxotrophic marker allowing for more potential integrations of the transposon cassette. This plasmid is expandable. Components: 1 vial containing 10µg of plasmid DNA | Map | $650 |
| pDMT4-3 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-3 has a native LEU2 promoter driving a LEU2 auxotrophic marker. This plasmid is expandable. Components: 1 vial containing 10µg of plasmid DNA | Map | $650 |
| pDMT4-4 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-4 has a defective LEU2 promoter driving a LEU2 auxotrophic marker allowing for more potential integrations of the transposon cassette. This plasmid is expandable. Components: 1 vial containing 10µg of plasmid DNA | Map | $650 |
| pDMT4-5 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-5 has a native HIS3 promoter driving a HIS3 auxotrophic marker. This plasmid is expandable. Components: 1 vial containing 10µg of plasmid DNA | Map | $650 |
| pDMT4-6 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-6 has a defective HIS3 promoter driving a HIS3 auxotrophic marker allowing for more potential integrations of the transposon cassette. This plasmid is expandable. Components: 1 vial containing 10µg of plasmid DNA | Map | $650 |
| pDMT4-7 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-7 has a native TRP1 promoter driving a TRP1 auxotrophic marker. This plasmid is expandable. Components: 1 vial containing 10µg of plasmid DNA | Map | $650 |
| pDMT4-8 | S. cerevisiae | Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-8 has a defective TRP1 promoter driving a TRP1 auxotrophic marker allowing for more potential integrations of the transposon cassette. This plasmid is expandable. Components: 1 vial containing 10µg of plasmid DNA | Map | $650 |
GGA=Golden Gate Assembly
Validated In Mammalian Cells,
Plants, And Yeast
Cas-CLOVER has been validated in mammalian cells, yeast for bioprocessing, and plants for crop trait engineering.
To learn more about the area you are interested in, visit the links below to the relevant resources.
Therapeutic Bioprocessing
Clean & clear CHO cell editing and GS CHO knockouts for bioprocessing
Agriculture
Advanced crop trait engineering, the non-GMO way
Industrial Biotechnology
Harnessing the power of targeted
yeast editing
Watch Our Videos
Enhance your gene editing research interests with Cas-CLOVER. The recorded webinars hosted by Genetic Engineering & Biotechnology News are available below so that you can watch the areas that apply to your research.
You can also watch our video on how Cas-CLOVER is a more efficient gene editing tool than CRISPR.
Tseten Jamling, PhD.
Scientist, BMS
Cas-CLOVER fundamentals, immunotherapy model creation, CHO cell editing, yeast, & plant trait development.
Maximilian Richter, PhD.
Senior Scientist,
Immuno-Oncology
Poseida Therapeutics
Advanced T-cell engineering to overcome known challenges with autologous CAR-T.
We Enable Researchers And Companies To Obtain More With Less Inconvenience
Contact us to learn more about our gene editing technology.