Optimize Your Gene Editing With The Powerful Toolbox Of Precision Cas-CLOVER™ And piggyBac® Gene Insertion Technologies

Shop Cas-CLOVER reagents, now available for risk free research evaluation and or commercial applications with full freedom to operate (FTO).

2D Cas-CLOVER-piggyBac Toolbox

Browse Reagents

In order to facilitate commercial development in the fields of Pharmaceutical Bioprocessing, Synthetic Biotechnology and Agricultural Biotechnology, we have removed barriers to entry typical of other gene editing technologies.

Our technology evaluation licensing process is risk free.

Simply purchase Cas-CLOVER or piggyBac reagents below and accept the simple evaluation agreement (which can also be downloaded and shared). We will provide our standard user protocols, which includes plasmid maps, instructions on design and production of vectors and gRNAs.

Demeetra - Shop - Workflow Icons - 1 - Order Reagents - Gene Editing Technology
1. Order Reagents
Demeetra - Shop - Workflow Icons - 2 - In-House or Outsource Evaluation Gene Editing Technology
2. Complete In-House Or Outsource Evaluation
Demeetra - Shop - Workflow Icons - 3 - Commercial License Gene Editing Technology
3. Get A Commercial License

Shop Cas-CLOVER & piggyBac Reagents

All prices include shipping & handling in the US. For International orders we will work with your shipping process and accounts.

Mammalian Gene Editing

Demeetra AgBio
Mammalian-Optimized
Cas-CLOVER mRNA
SKU#TechnologyDescriptionResourceAdd to cart
rDMT5-011Cas-CLOVER

Cas-CLOVER mRNA: Codon optimized research grade mRNA for mammalian systems. Use our gRNA pair design overview or Demeetra can custom design the target pairs for you (contact us below). Order gRNAs from your favorite provider and transform with the mRNA for high efficiency and clean editing.

Components: 1 vial containing 25µg of mRNA

Design Guide$650
Demeetra AgBio
Mammalian piggyBac Reagents
SKU#TechnologyDescriptionResourceAdd to cart
rDMT5-027piggyBac

Super piggyBac transposase mRNA: Codon optimized for mammalian systems. Transform cells with piggyBac mRNA and piggyBac transposon (pDMT5-028) for stable integration. Use for any cell of interest for biomanufacturing, food products, agriculture and synthetic biology.

Components: 1 vial containing 25µg of mRNA

User Guide$650
rDMT5-020piggyBac

Excision-only piggyBac transposase mRNA: Use Excision-only piggyBac transposase to remove previously integrated piggyBac transposons for phenotype reversions and Footprint-Free gene editing.

Components: 1 vial containing 25µg of mRNA

User Guide$650
pDMT5-029piggyBac

Super piggyBac transposase plasmid: Codon optimized for mammalian systems. Transform cells with piggyBac plasmid and piggyBac transposon (pDMT5-028) for stable integration. Use for any cell of interest for biomanufacturing, food products, agriculture and synthetic biology. Please note this plasmid is not expandable.

Components: 1 vial containing 25µg of plasmid DNA

Map

User Guide
$650
pDMT5-030piggyBac

Excision-only piggyBac transposase DNA: Use Excision-only piggyBac transposase to remove previously integrated piggyBac transposons for phenotype reversions and Footprint-Free gene editing. Please note this plasmid is not expandable.

Components: 1 vial containing 25µg of plasmid DNA

Map

User Guide
$650
pDMT5-028piggyBac

piggyBac transposon: Using the flexible MCS, clone in your promoter, gene and other components for optimal expression in your system. This plasmid is expandable.

Components: 1 vial containing 10µg of plasmid DNA

Map$350

Cas-CLOVER gRNA Design Services

Demeetra AgBio - Shop - Cas-CLOVER gRNA Design Services
Cas-CLOVER gRNA Design Services
SKU: D003

Expert consultation and design deliverable for one pair (2x gRNAs) using state-of-the-art methods gene editing with Cas-CLOVER

1 Pair (2x gRNAs designed)

Plant Gene Editing

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Plant Cas-CLOVER Gene Editing Plasmid Vector Suite

Codon optimized T-DNA based plant transformation vectors for gene knockout in plants using the Cas-CLOVER system. Expression promoters for Cas-CLOVER, gRNA’s, selection markers and other details are listed below along with links to the maps.

All vectors are 5µg vial

Plasmid Sku#Codon OptimizationCas-CLOVER PromotergRNA PromoterHost SelectionBacterial SelectionPlasmid NotesMap linkAdd to cart
pDMT1-1A. thalianaCaMV35SOsU3HygromycinKanamycinSequential cloning of gRNAsMap$500
pDMT3-4A. thalianaCaMV35SAtU6-26HygromycinKanamycinSequential cloning of gRNAsMap$500
pDMT1-123A. thalianaCaMV35SOsU6HygromycinKanamycinSequential cloning of gRNAsMap$500
pDMT1-112A. thalianaCaMV35SOsU3KanamycinKanamycinSequential cloning of gRNAsMap$500
pDMT1-114A. thalianaCaMV35SAtU6-26KanamycinKanamycinSequential cloning of gRNAsMap$500
pDMT1-116A. thalianaCaMV35SOsU3GlufosinateKanamycinSequential cloning of gRNAsMap$500
pDMT1-118A. thalianaCaMV35SAtU6-26GlufosinateKanamycinSequential cloning of gRNAsMap$500

GGA=Golden Gate Assembly

Yeast Gene Editing

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Yeast Cas-CLOVER Gene Editing
Vector Suite

We offer a series of yeast-based shuttle vectors for the expression of a Saccharomyces cerevisiae codon optimized version of Cas-CLOVER. In this platform we have designed the plasmids such that gRNA expression is polyscistronic. Expression of the Cas-CLOVER is driven by ScRNR2 promoter, and individual guides are expressed by ScSNR52 and are liberated via an intervening tRNA.

Plasmid Sku#Codon OptimizationCas-CLOVER PromotergRNA PromoterHost SelectionHost ReplicationBacterial SelectionNotes About PlasmidMap linkAdd to cart
pDMT1-124S. cerevisiaeScRNR2No Guide Expression CassetteLEU2CEN/ARSAmpicillinBsaI sites have been mutated.Map$500
pDMT1-125S. cerevisiaeScRNR2ScSNR52LEU2CEN/ARSAmpicillinTwo guides can be cloned via GGAMap$500
pDMT1-126S. cerevisiaeScRNR2ScSNR52URA3CEN/ARSAmpicillinTwo guides can be cloned via GGAMap$500
pDMT1-127S. cerevisiaeScRNR2ScSNR52KanRCEN/ARSAmpicillinTwo guides can be cloned via GGAMap$500
pDMT1-128S. cerevisiaeScRNR2ScSNR52HygRCEN/ARSAmpicillinTwo guides can be cloned via GGAMap$500

GGA=Golden Gate Assembly

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Yeast piggyBac Engineering Vector Suite
Plasmid SKU#Codon OptimizationDescriptionMap linkAdd to cart
pDMT3-23S. cerevisiae

Taking advantage of the unique massive cargo capacity, this ~25kb piggyBac transposon vector contains all genetic components required for engineering the yeast mevalonate pathway (MVA) in a single-step for the production of a high value secondary metabolites. This plasmid is expandable. View data<.a>.

Components: 1 vial containing 10µg of plasmid DNA

Map$1998
pDMT4-1S. cerevisiae

Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-1 has a native URA3 promoter driving a URA3 auxotrophic marker. This plasmid is expandable

Components: 1 vial containing 10µg of plasmid DNA

Map$650
pDMT4-2S. cerevisiae

Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-2 has a defective URA3 promoter driving a URA3 auxotrophic marker allowing for more potential integrations of the transposon cassette. This plasmid is expandable.

Components: 1 vial containing 10µg of plasmid DNA

Map$650
pDMT4-3S. cerevisiae

Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-3 has a native LEU2 promoter driving a LEU2 auxotrophic marker. This plasmid is expandable.

Components: 1 vial containing 10µg of plasmid DNA

Map$650
pDMT4-4S. cerevisiae

Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-4 has a defective LEU2 promoter driving a LEU2 auxotrophic marker allowing for more potential integrations of the transposon cassette. This plasmid is expandable.

Components: 1 vial containing 10µg of plasmid DNA

Map$650
pDMT4-5S. cerevisiae

Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-5 has a native HIS3 promoter driving a HIS3 auxotrophic marker. This plasmid is expandable.

Components: 1 vial containing 10µg of plasmid DNA

Map$650
pDMT4-6S. cerevisiae

Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-6 has a defective HIS3 promoter driving a HIS3 auxotrophic marker allowing for more potential integrations of the transposon cassette. This plasmid is expandable.

Components: 1 vial containing 10µg of plasmid DNA

Map$650
pDMT4-7S. cerevisiae

Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-7 has a native TRP1 promoter driving a TRP1 auxotrophic marker. This plasmid is expandable.

Components: 1 vial containing 10µg of plasmid DNA

Map$650
pDMT4-8S. cerevisiae

Customizable piggyBac transposon vector containing a large multiple cloning site (MCS) flanked by piggyBac ITRs for integration and core insulators which protect against gene silencing. pDMT4-8 has a defective TRP1 promoter driving a TRP1 auxotrophic marker allowing for more potential integrations of the transposon cassette. This plasmid is expandable.

Components: 1 vial containing 10µg of plasmid DNA

Map$650

GGA=Golden Gate Assembly

Validated In Mammalian Cells,
Plants, And Yeast

Cas-CLOVER has been validated in mammalian cells, yeast for bioprocessing, and plants for crop trait engineering.

To learn more about the area you are interested in, visit the links below to the relevant resources.

Demeetra AgBio

Therapeutic Bioprocessing

Clean & clear CHO cell editing and GS CHO knockouts for bioprocessing

Demeetra AgBio
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Agriculture

Advanced crop trait engineering, the non-GMO way

Demeetra AgBio
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Industrial Biotechnology

Harnessing the power of targeted
yeast editing

Demeetra AgBio

Watch Our Videos

Enhance your gene editing research interests with Cas-CLOVER. The recorded webinars hosted by Genetic Engineering & Biotechnology News are available below so that you can watch the areas that apply to your research.

You can also watch our video on how Cas-CLOVER is a more efficient gene editing tool than CRISPR.

Demeetra AgBio

Tseten Jamling, PhD.

Scientist, BMS

Cas-CLOVER fundamentals, immunotherapy model creation, CHO cell editing, yeast, & plant trait development.

Demeetra AgBio

Maximilian Richter, PhD.

Senior Scientist,
Immuno-Oncology
Poseida Therapeutics

Advanced T-cell engineering to overcome known challenges with autologous CAR-T.

We Enable Researchers And Companies To Obtain More With Less Inconvenience

Contact us to learn more about our gene editing technology.