Optimize Your Gene Editing with Cas-CLOVER Technology
Shop Cas-CLOVER reagents, now available for research or commercial applications.
Are you conducting gene editing in cells, animals, plants or yeast for a non-profit or commercial organization? Would you like the opportunity to commercialize the gene edited products, or traits?
Our Cas-CLOVER gene editing system “the clean alternative to CRISPR-Cas9” is now available for purchase for commercial and non-profit use. In order to remove restrictions typical of other gene editing technologies, our MTA includes a path to commercialization of materials produced by your lab (in our joint fields of use including: research, drug discovery, bioprocessing, agriculture & industrial biotechnology).
Simply complete your purchase of Cas-CLOVER reagents below with the option for us to design your guides. We will provide our standard use protocol, which includes maps, sequences, instructions on design and production of the gRNAs.
All reagent prices include shipping & handling. The mammalian mRNA product requires shipping on dry ice and costs more to ship.
Shop Cas-CLOVER Reagents
Mammalian gene editing
Mammalian Codon Optimized Cas-CLOVER mRNA
Improved mRNA stability and protein expression in mammalian systems. Order custom designed and produced gRNAs from us (see options below) or your favorite provider and transform with the mRNA for clean editing.
Plant gene editing
Plant Cas-CLOVER Gene Editing Plasmid Vector Suite
T-DNA based plant transformation vectors for gene knockout in plants using the Cas-CLOVER system. The system is codon optimized for plant expression. Expression promoters for Cas-CLOVER, gRNA’s, selection markers and other details are listed below along with links to the maps.
All vectors are 5µg vial
|Plasmid Sku#||Codon Optimization||Cas-CLOVER Promoter||gRNA Promoter||Host Selection||Bacterial Selection||Plasmid Notes||Map link||Add to cart|
|pDMT1-1||A. thaliana||CaMV35S||OsU3||Hygromycin||Kanamycin||Sequential cloning of gRNAs||Map||$99|
|pDMT3-4||A. thaliana||CaMV35S||AtU6-26||Hygromycin||Kanamycin||Sequential cloning of gRNAs||Map||$99|
|pDMT1-123||A. thaliana||CaMV35S||OsU6||Hygromycin||Kanamycin||Sequential cloning of gRNAs||Map||$99|
|pDMT1-112||A. thaliana||CaMV35S||OsU3||Kanamycin||Kanamycin||Sequential cloning of gRNAs||Map||$99|
|pDMT1-114||A. thaliana||CaMV35S||AtU6-26||Kanamycin||Kanamycin||Sequential cloning of gRNAs||Map||$99|
|pDMT1-116||A. thaliana||CaMV35S||OsU3||Glufosinate||Kanamycin||Sequential cloning of gRNAs||Map||$99|
|pDMT1-118||A. thaliana||CaMV35S||AtU6-26||Glufosinate||Kanamycin||Sequential cloning of gRNAs||Map||$99|
|pDMT1-136||A. thaliana||CaMV35S||AtU6-26-Two guide system||Hygromycin||Kanamycin||Two guides cloned via GGA||Map||$99|
|pDMT1-137||A. thaliana||CaMV35S||OsU3-Two guide system||Hygromycin||Kanamycin||Two guides cloned via GGA||Map||$99|
|pDMT1-138||A. thaliana||CaMV35S||OsU6-Two guide system||Hygromycin||Kanamycin||Two guides cloned via GGA||Map||$99|
|pDMT1-142||A. thaliana||CaMV35S||AtU6-26-Two guide system||Kanamycin||Kanamycin||Two guides cloned via GGA||Map||$99|
|pDMT1-143||A. thaliana||CaMV35S||OsU3-Two guide system||Kanamycin||Kanamycin||Two guides cloned via GGA||Map||$99|
|pDMT1-144||A. thaliana||CaMV35S||OsU6-Two guide system||Kanamycin||Kanamycin||Two guides cloned via GGA||Map||$99|
|pDMT1-145||A. thaliana||CaMV35S||AtU6-26-Two guide system||Glufosinate||Kanamycin||Two guides cloned via GGA||Map||$99|
|pDMT1-146||A. thaliana||CaMV35S||OsU3-Two guide system||Glufosinate||Kanamycin||Two guides cloned via GGA||Map||$99|
|pDMT1-147||A. thaliana||CaMV35S||OsU6-Two guide system||Glufosinate||Kanamycin||Two guides cloned via GGA||Map||$99|
GGA=Golden Gate Assembly
Yeast Gene Editing
Yeast Cas-CLOVER Gene Editing Vector Suite
We offer a series of yeast-based shuttle vectors for the expression of a Saccharomyces cerevisiae codon optimized version of Cas-CLOVER. In this platform we have designed the plasmids such that gRNA expression is polyscistronic. Expression is driven by ScDMPR1, a proprietary promoter (information will be provided following your purchase), and individual guides are liberated via an intervening tRNA. Plasmids are offered on low copy CEN/ARS origin of replication.
|Plasmid Sku#||Codon Optimization||Cas-CLOVER Promoter||gRNA Promoter||Host Selection||Host Replication||Bacterial Selection||Notes About Plasmid||Map link||Add to cart|
|pDMT1-124||S. cerevisiae||ScDMPR1||No Guide Expression Cassette||LEU2||CEN/ARS||Ampicillin||BsaI sites have been mutated.||Map||$99|
|pDMT1-125||S. cerevisiae||ScDMPR1||ScSNR52||LEU2||CEN/ARS||Ampicillin||Two guides can be cloned via GGA||Map||$99|
|pDMT1-126||S. cerevisiae||ScDMPR1||ScSNR52||URA3||CEN/ARS||Ampicillin||Two guides can be cloned via GGA||Map||$99|
|pDMT1-127||S. cerevisiae||ScDMPR1||ScSNR52||KanR||CEN/ARS||Ampicillin||Two guides can be cloned via GGA||Map||$99|
|pDMT1-128||S. cerevisiae||ScDMPR1||ScSNR52||HygR||CEN/ARS||Ampicillin||Two guides can be cloned via GGA||Map||$99|
GGA=Golden Gate Assembly
Cas-CLOVER gRNA Design & Production Services
Cas-CLOVER gRNA Design Services
Synthesis of 1 gRNA using state-of-the-art methods to be used for gene editing with Cas-CLOVER
Custom gRNA Production Services
Synthesis of 1 pair (two gRNAs) using state-of-the-art methods to be used for gene editing with Cas-CLOVER
1 pair gRNA
Validated in CHO Cells, Yeast, Plants & Human T-Cells
Cas-CLOVER has been validated in CHO cell lines and yeast for bioprocessing, plants for crop trait engineering and human T-cells for immunotherapy research..
To learn more about the area you are interested in, download the relevant industry datasheet.
Drug Discovery & Development
Advancing cell-line engineering for pharmaceutical research
Clean & clear CHO cell editing and GS CHO knockouts for bioprocessing
Advanced crop trait engineering, the non-GMO way
Harnessing the power of targeted yeast editing
Watch Our Videos
Enhance your gene editing research interests with Cas-CLOVER. The recorded webinars hosted by Genetic Engineering & Biotechnology News are available below so that you can watch the areas that apply to your research.
You can also watch our video on how Cas-CLOVER is a more efficient gene editing tool than CRISPR.
Tseten Jamling, PhD.
R&D and Gene Editing
Cas-CLOVER fundamentals, immunotherapy model creation, CHO cell editing, yeast, & plant trait development.
Maximilian Richter, PhD.
Advanced T-cell engineering to overcome known challenges with autologous CAR-T.
Get a Jump Start On Your Gene Editing
Procure Cas-CLOVER reagents by our easy-to-use shop page by simply purchasing the vials and signing a simple royalty-free MTA form.
Shortly after your purchase, you’ll get our basic use protocol, which includes maps, sequences, instructions on design and production of the gRNAs.
Get started on your gene editing today.
We Enable Researchers and Companies To Obtain More With Less Inconvenience
Contact us to learn more about our gene editing technology.
DEMEETRA AGBIO, INC.
2277 THUNDERSTICK DR. #500 LEXINGTON, KY 40505 US
COPYRIGHT © 2020 DEMEETRA AGBIO, INC. - ALL RIGHTS RESERVED.