Cas-CLOVER™: Gene Editing More Precise Than CRISPR

Cas-CLOVER gene editing technology

Contact us to learn more about our gene editing technology.

Cas-CLOVER gene editing technology

If you have experienced unwanted off-target mutations or confusing licensing and freedom to operate issues when using gene editing tools like CRISPR, you are not alone.

Demeetra AgBio - Shop - Cas-CLOVER gRNA Design Services

Demeetra is here to help.

Our Cas-CLOVER gene editing technology has undetectable off-target activity and stand alone patent protection for licensing simplicity and clarity in freedom to operate.

Evaluate Cas-CLOVER in-house or collaborate with us to execute your proof of concept services. Then, partner with us for accessible commercial and research licenses for the areas of agriculture, industrial biotechnology and pharmaceutical bioprocessing for a more rewarding gene editing experience.

Contact us to learn more.

Validated In CHO cells, Yeast and Plants

The Cas-CLOVER system has been validated in Chinese hamster ovary (CHO) cell lines and yeast for bioprocessing, as well as plants for crop trait engineering.

To learn more about the area you are interested in, download the relevant industry datasheet.

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Therapeutic Bioprocessing

Clean & clear CHO cell editing and GS CHO knockouts for bioprocessing

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Agriculture

Advanced crop trait engineering, the non-GMO way

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Industrial Biotechnology

Harnessing the power of targeted yeast editing

Cas-CLOVER gene editing technology
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What’s Unique About The Cas-CLOVER Gene Editing System?

As opposed to the use of a single guide RNA (gRNA) for sequence-specific gene editing, the Cas-CLOVER system utilizes a dual gRNA in addition to the Clo051 nuclease activity that requires dimerization of subunits associated with each guide RNA.

This allows for more stringent DNA cleavage. Using two gRNA makes the Cas-CLOVER gene editing system highly restricted and only functional when the paired gRNAs coexist.

Freedom To Operate Is Clear With Cas-CLOVER

The lack of clear commercial freedom to operate and use of intellectual property (IP) has limited the commercial use of current gene editing tools. It is our mission to make technology easily accessible to all scientists and researchers within the pharmaceutical bioprocessing, industrial biotechnology, and agriculture industries.

Due to the distinct science involved, Cas-CLOVER is covered under a set of patents separate from other technologies. Cas-CLOVER is exclusively licensed and sub-licensable by Demeetra.

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Introduction to Cas-CLOVER

Enhance your gene editing research or commercial interests with Cas-CLOVER. Watch to see the unique ways Cas-Clover can work for you.

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Browse Cas-CLOVER Gene Editing Reagents

Browse Cas-CLOVER reagents for use in mammalian, plant, and yeast gene editing by visiting our easy-to-use catalog page. After ordering the vials and signing a simple royalty-free MTA form, we’ll send you our basic use protocol, which includes maps; sequences; and instructions on design and production of the gRNAs.

Scientists Trust Our Gene Editing Technology

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We are very happy with the efficiency of the targeted mutations we are getting with Cas-CLOVER in mammalian cells. It is impressive that although Cas-CLOVER requires dimerization it still has very high efficiency similar to CRISPR/Cas9. The one aspect of Cas-CLOVER that stood out for us was its ease of use. We have been working with CRISPR/Cas9 for many year now and Cas-CLOVER just fit in very nicely with our existing workflow for gene-editing with Cas9 or Cas9 nickase.

Demeetra AgBio

Tseten Jamling, PhD.

VP, R&D & Gene Editing
Hera BioLabs

Demeetra AgBio

Cas-CLOVER’s ability to edit resting primary T cells with efficiency and fidelity allows us to generate fully allogeneic CAR-T products rich in TSCM cells.

Demeetra AgBio

Maximilian Richter, PhD.

Senior Scientist,
Immuno-Oncology
Poseida Therapeutics

We Enable Scientists To Obtain More With Less Inconvenience

Contact us to learn more about our gene editing technology.

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