Cas-CLOVER And Its Advantages Over Nobel Award-Winning CRISPR/Cas9
Emmanuelle Charpentier and Jennifer Doudna were awarded the 2020 Nobel Prize in Chemistry for discovering CRISPR/Cas9 ‘genetic scissors’ for editing DNA in an in vitro system, published in Science back in June 2012 (1). CRISPR/Cas9 is derived from a bacterial immune system in which guide RNAs carry the Cas9 protein to cut out specific regions…
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piggyBac: A Powerful Tool For Insertional Mutagenesis
While CRISPR based technologies such as Cas-CLOVER can cut DNA with targeted precision, the insertion of very large transgenes or seamless removal of genes, particularly in uncharacterized genomes, necessitates additional tools. The Super piggyBac transposase system employs a transposase/transposon mechanism to introduce or excise genes of varying sizes into host DNA. Furthermore, piggyBac transposase facilitates…
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Gene Editing for Long-Lasting Fresh Tomatoes
In contemporary society, the practice of shopping on a weekly rather than daily basis presents challenges in maintaining the freshness of produce. Preserving produce is critical for economic efficiency and mitigating greenhouse gas emissions resulting from food waste. Advanced gene editing technology facilitates precise targeted mutations without the introduction of transgenes, enabling the deactivation of…
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