Demeetra AgBio, Inc.

piggyBac Transposase in Non-Conventional Yeasts: Metabolites and Small Molecules

By Demeetra AgBio, Inc. / January 15, 2021

Yarrowia lipolytica has been one of the most relevant yeast strains for synthetic biology in recent years, with many studies showing promise for its increased capacity in bioprocessing. There’s only one constraint – having essential genetic modification tools necessary to become a fully utilized yeast platform at an industrial scale. Non-conventional Y. lipolytica is currently…

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Larger Deletions, Easier Screening with Demeetra’s Cas-CLOVER Technology

By Demeetra AgBio, Inc. / January 11, 2021

Demeetra’s Cas-CLOVER dimeric gene editing system has been found to result in easier screening, higher knockout mutation percentages and flexible target design. Recently Demeetra and Elanco Animal Health presented a webinar together on collaborative data in gene editing using Cas-CLOVER for cell line development in bioprocessing of biologic therapeutics. Two key points were discussed with…

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Increasing Cutting Efficiency in Tobacco with the Cas-CLOVER System

By Demeetra AgBio, Inc. / December 17, 2020

The CRISPR-Cas9 system has been an effective technology for targeted genome engineering with the capability to efficiently edit a vast array of organisms. However, CRISPR-Cas9 also has a few major disadvantages, including off-target mutations and licensing restrictions. In recent years, these drawbacks have created multiple challenges with upscaling to a commercial product with this particular…

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Cas-CLOVER And Its Advantages Over Nobel Award-Winning CRISPR/Cas9

By Demeetra AgBio, Inc. / November 18, 2020

Emmanuelle Charpentier and Jennifer Doudna have been awarded this year’s Nobel Prize in Chemistry for discovering CRISPR/Cas9 ‘genetic scissors’ for editing DNA in an in vitro system in Science back in June 2012. CRISPR/Cas9 is derived from a bacterial immune system in which strips of guide RNA carry the Cas9 protein to cut out specific…

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piggyBac: a Powerful Tool for Insertional Mutagenesis

By Demeetra AgBio, Inc. / November 3, 2020

CRISPR may get all the attention for being able to cut DNA in a directed way, but how do researchers insert or remove genes or what if the gene(s) that produce phenotypes of interest are not known? The piggyBac (PB) system can be used for the introduction of genes into many different organisms. These genes…

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Improving Protein Production in Tobacco With Gene Editing

By Demeetra AgBio, Inc. / September 24, 2020

Like yeasts, E. coli and other microorganisms, plants have long been useful tools to produce recombinant proteins through genetic modifications. The goals of these modifications vary from producing therapeutic proteins to making proteins for industrial use. However, genetically altering organisms to produce a protein of interest is a time consuming and labor intensive process, but…

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Modifying Tobacco Plants To Reduce Cancer Risk For Various Ethnic Groups Possible With Gene Editing

By Demeetra AgBio, Inc. / September 24, 2020

The link between smoking and cancer is well established; however, genetic variation leading to differential health outcomes across ethnic populations isn’t as clear. Genes Associated With Lung Cancer Research has shown that CYP2A6 and CYP2A13 gene variants are associated with a reduced risk of lung cancer among smokers of diverse ethnic backgrounds. These genes fall…

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Decreasing Carcinogen Levels in Tobacco With Gene Editing

By Demeetra AgBio, Inc. / September 1, 2020

Tobacco has been cultivated for hundreds of years and a source of income for many around the world. It’s not only found in the modern day commercially sold cigarette that includes several other ingredients besides tobacco, but is also consumed in the form of cigars and hookahs. And, some even roll their own tobacco which…

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Seedless Fruit Production With Gene Editing

By Demeetra AgBio, Inc. / August 20, 2020

Parthenocarpy (the production of seedless fruit without prior fertilization) occurs in nature whether through a mutation that leads to negative consequences or as a benefit to shield against predators. It can also be exploited by scientists to achieve produce with longer shelf- lives or for practical purposes; such as large scale sauce production, or more…

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The Significance of Genetically Modifying Glutamate in Plants

By Demeetra AgBio, Inc. / August 19, 2020

As the population continues to grow and food production becomes more difficult due to weather patterns, disease, and lack of nutrient rich land, better solutions are needed. It is the hope of many scientists all over the world that genetically modifying plants and animals will assist with the challenges that we face, specifically wipe out…

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