Harbor-IN: License-Free Transposase for High-Titer Biologics Production

Random plasmid integration can leave cell lines with unstable genomes and lower productivity. Harbor-IN is a license-free transposase system that integrates genes at high efficiency into genomic regions optimized for robust, sustained expression.

Harbor-IN options

Harbor-IN. Now available in two formats.

Choose the standard Harbor-IN mRNA format or Harbor-IN Elite, a premium animal-origin-free format with documented material-origin and TSE/BSE support.

Standard format

Harbor-IN

License-free transposase mRNA for stable, high-efficiency gene integration in biologics cell line development. Used by dozens of biologics CLD teams globally and manufactured with only limited animal-origin materials from sources considered negligible risk.

Premium format

Harbor-IN Elite

The Harbor-IN integration system supplied as non-animal, non-human-origin mRNA, with manufacturing materials documented as synthetic or plant origin.

Current pricing and purchase options are maintained on Demeetra’s Products & Pricing page.

Unlock the full potential of biologics manufacturing

Built for stable, high-productivity cell line development

Harbor-IN is uniquely suited for bioproduction, helping teams establish stable expression while reducing reliance on labor-intensive single-cell cloning and process intensification strategies.

Up to 18 g/L

Demonstrated mAb titer

3 Proven Formats

mAbs • bsAbs • Fc-fusions

60+ Generations

Stable in Pools & Clones

Commercial access

License-free commercial use: freedom to operate

Harbor-IN is designed to remove licensing friction from stable cell line development and bioprocessing.

Unlike transposase systems that can require separate licensing negotiations, Harbor-IN is available for commercial use through the product purchase model. Companies can use the technology for cell line development and bioprocessing without an additional platform licensing fee.

Harbor-IN purchase model
Technology access that stays straightforward
  • Research, development and commercial bioprocessing use
  • No separate platform license required
  • No additional Harbor-IN licensing fee layered onto product access
  • Compatible with CHO and HEK293 stable cell line workflows
How Harbor-IN works

Stable integration where expression can perform

Harbor-IN integrates genetic material into accessible genomic regions, favoring highly transcribed, open chromatin for robust and sustained gene expression.

Harbor-IN is shown favoring accessible, unfolded DNA, compared with random integration into chromatin-packed chromosome regions.

STEP 01

Co-transfect

Deliver Harbor-IN mRNA with a transposon carrying the expression cassette.

STEP 02

Transient expression

Harbor-IN transposase is expressed transiently from the mRNA.

STEP 03

Stable integration

The expression cassette integrates into the host genome.

STEP 04

Evaluate performance

Advance stable pools into productivity assessment and clone selection.

Harbor-IN Starter Kit

Fast-track your evaluation

The Harbor-IN Starter Kit combines transposase mRNA with optimized DNA transposons so teams can evaluate the system in an end-to-end stable integration workflow.

View Starter Kit Pricing
  • 1
    Harbor-IN transposase mRNA

    25 µg mRNA format for stable genomic integration workflows.
  • 2
    Optimized DNA transposons

    A set of preconfigured transposons to support evaluation, selection and expression workflows.
  • 3
    Cloning flexibility

    Transposon designs support insertion of user-defined expression cassettes.
Harbor-IN Elite

Animal-origin-free transposase mRNA, built into the Harbor-IN workflow

Elite is the premium Harbor-IN format for programs that value completely animal-origin-free manufacturing materials and associated quality controls at the cell line development stage. It retains the Harbor-IN integration approach while using synthetic or plant-origin manufacturing inputs supported by corresponding material-origin and quality documentation.

What Elite adds
Documented non-animal, non-human-origin manufacturing inputs

All raw materials, reagents and processing aids used to manufacture the Elite mRNA are synthetic or plant origin.

01

Animal-origin-free

No materials of animal or human origin are used in the documented manufacturing process for the supplied Elite lot.

02

ISO 7 cleanroom alignment

Manufactured in the United States under controlled conditions aligned with best laboratory practices in an ISO 7 cleanroom.

03

TSE/BSE risk negligible

Based on manufacturing assessment documentation, TSE/BSE contamination risk is considered negligible.

Harbor-IN Elite delivers comparable integration performance in CHO cells

Experimental comparison of Standard Harbor-IN and Harbor-IN Elite in suspension CHO cells transfected with a GFP expression transposon and analyzed without GS selection.

GFP% Cells
020406080100 GFP+ Cells (%) Mock Harbor-IN Harbor-IN Elite
Average Copy Number in Unselected Pools (dPCR)
0246810 Copies / Cell Mock Harbor-IN Harbor-IN Elite

Harbor-IN and Harbor-IN Elite gave comparable GFP-positive pools and comparable transgene copy number by dPCR. Selection would raise both metrics further.

Elite quality documentation

Documentation available for customer review

Demeetra’s supplier package substantiates the Elite material-origin position and provides lot-specific analytical information.

Certificate of Analysis

Lot-specific results include appearance, pH, concentration, A260/280, endotoxin and fragment-analysis information. The supplied lot passed the reported acceptance criteria.

Statement of Origin

Documents U.S. manufacture, ISO 7 cleanroom alignment, and non-animal, non-human origin, with manufacturing materials identified as synthetic or plant origin.

TSE/BSE Statement

States that no animal- or human-origin materials are used in the manufacturing process and that TSE/BSE contamination risk is negligible.

Product-use note: Supplier documentation identifies the Harbor-IN Elite mRNA itself as Research Use Only and not approved for clinical, diagnostic or therapeutic use in humans or animals.

Combine Harbor-IN with Demeetra biologics expression platforms

One integration technology, multiple ways to build the production cell line

Harbor-IN can be paired with Demeetra’s engineered CHO platforms and genome-editing capabilities to support stable expression, targeted cell engineering and biologics development.

CleanCut GS CHO

Pair Harbor-IN stable integration with Demeetra’s GS-engineered CHO platform for biologics cell line development and production workflows.

Cas-CLOVER

Add targeted nuclease editing when the development strategy requires precise knockouts or site-directed genomic modifications.

Choose the Harbor-IN format that fits your program.

Talk with Demeetra about Standard Harbor-IN, Harbor-IN Elite, starter kits, protocols and how the technology fits your cell line development workflow. Current product pricing and purchase options are available through Products & Pricing.

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Products & Pricing