Demeetra utilizes Cas-CLOVER as our go-to gene editing technology for many reasons. Cas-CLOVER is simple to design and build and has extremely high efficiencies and target fidelity. For every gene we have attempted to edit, we have found ample Cas-CLOVER anchor binding sites.
However, the requirement of a GG “PAM” binding site is technically a constraint on Cas-CLOVER and other gRNA-dependent biotechnologies like CRISPR-Cas9.
TAL-CLOVER remedies this since it only requires a “T” anchor. This results in more plentiful anchor binding sites especially in TA-rich regions of the genome. Another advantage is that TAL-CLOVER, like Cas-CLOVER, requires enzyme dimerization and thus has high fidelity.
The main downside is that the TALs in TAL-CLOVER are large, generally hard to build, and labor intensive. But we have perfected the art and routinely generate TAL pairs in just 10 days. Now, we are offering this version of our gene editing technology as an option to our licensees.
Figure 1: breakdown of some features of Cas-CLOVER and TAL-CLOVER
Like one of our Cas-CLOVER validation studies, TAL-CLOVER was used for markerless disruption of a pathway in yeast, resulting in the accumulation of red pigment in vacuole.
Figure 2: Petri dish showing the accumulation of red pigment in vacuole
If you’re interested in learning more about how Cas-CLOVER, take a look here for additional details. Visit our shop as we have everything for your gene editing needs, including flexible front or back-loaded service terms and licensing options.