Stable Gene Expression With piggyBac

The proven piggyBac transposase system can deliver high-yield protein production, flexibly edit any genome, rapidly screen novel strains, amplify synthetic biotechnology, and more.

Clean

Non-viral
and stable.

Flexible

Performs small to large gene integrations (200KB+)

Efficient

Cost-effective; features high expression of introduced genes.

"Cut & Paste" With piggyBac Transposase + Transposon

The piggyBac transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and cuts them from the original vector. PiggyBac then integrates the expression cargo flanked by the ITRs into non-coding open chromosomal sites. This allows for the stable integration of the genes of interest at highly expressed sites in the genome.

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Flexible Range Of Cargo

The piggyBac transposase can integrate a wide range of DNA fragment sizes, from small gene fragments up to entire metabolic pathways containing several genes (200 kb+).

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A Proven Technology

The piggyBac platform has been published over 750 times with many different applications. In addition to generating cell lines with highly expressed and stable gene integration, piggyBac has been utilized to rapidly develop mutant libraries for loss and gain of function across multiple species. The integrated ITRs enable rapid mapping of the introduced genes.

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Validated In Mammalian Cells, Yeast, and Plants

Our expertise and field of use spans multiple industries, allowing for the integration of Cas-CLOVER and transposase technologies into a wide range of commercial applications.