Amplified Synthetic Biotechnology

Due to their exceptional natural characteristics, yeasts and other microbes play an important role as the platform for bioproduction of biofuels, therapeutics, food ingredients, and proteins.

By applying Cas-CLOVER and piggyBac gene editing technology to microbial strain engineering, these traits can be further leveraged for commercial manufacturing, product discovery, and development.

A clean alternative to CRISPR/Cas9, Cas-CLOVER has the potential to revolutionize synthetic biotechnology.

It utilizes a dimeric Clo051 nucleus domain recruited by guide RNAs (gRNAs). The pair of gRNAs and dimeric architecture significantly remove the risk of off-targets compared to Cas9 and other gene editing technologies.

Known for high efficiency and stable, high expression; piggyBac has demonstrated activity in a diversity of yeast strains, including many non-model organisms for commercial applications – per published data.

Small to very large stable gene integration (200KB+) enables whole pathway metabolic engineering and high expression of proteins. In this example, we integrated the Mevalonate Pathway (approximate 30 kb) with piggyBac and saw an almost 9-fold increase in β-Farnesene production with no optimization of growth conditions.