Landing Pad Methods for Next-Generation Cell Line Development
We are releasing a new white paper demonstrating that Cas-CLOVER enables efficient, precise knock-ins in our CleanCut GS CHO cell host, establishing a robust foundation for next-generation cell line development and bioprocessing.
Key results from the study:
In this work, we used a targeted knock-in at the endogenous GAPDH locus as a quantitative readout for editing efficiency. Using Cas-CLOVER, we observed the emergence of a >20% stable, GFP-only knock-in population without selection, indicating highly efficient and precise targeted integration.
To demonstrate broader applicability, we also targeted Chr6-AttP and C12orf35, two commonly used CHO safe harbor sites. At both loci, precise HDR-mediated integrations were confirmed, demonstrating that Cas-CLOVER performs reliably beyond a single genomic context.
Why this matters for CLD and bioprocessing: Cas-CLOVER–edited CleanCut GS cells support the programmable installation of recombinase or integrase landing pads, enabling:
- Single-copy or multi-copy insertion strategies
- Orthogonal landing pad architectures
- Reduced clone-to-clone variability
- Lower screening burden and faster CLD timelines
- Clearer regulatory pathways compared to random integration